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HEMOGOLOBIN ELECTROPHORESIS ۱) ACCESSORIES – Chamber – Applicator Kit – Staining Set and Rack ۲) REAGENTS AND CONSUMABLES – Acetate plates TITAN IV H – Super Hema buffer ( 1bag diluted in 1000 ml distilled water ) – Ponceau S (1bag diluted in 1000 ml distilled water ) – Clear Aid – A1 SA2 – A1FSA2 – A1FSC – Hemolysate reagent – Report Forms – Blotters – Methanol – Glacial acetic acid . ۳) METHOD – Wet the plates 15mn in buffer – One cathode application ۴) STAINING – Ponceau S : 3 mn . – Deshydratation : 2 diffrents washes in pure methanol 2 mn each . – Clearing : 10 mn in the clearing solution . – Clearing solution ………….. ۶۷ % pure methanol – At the end of the clearing time drain the plate vertically for about 1 mn . – Wipe the mylar side . ۵) SCANNING – Scan with your densitometer at 525 mn . LIPOPROTEINS ELECTROPHORESIS ۱) ACCESSORIES – Power Supply – Chamber – Applicator Kit ۲) REAGENTS AND CONSUMABLES – Acetate plates – HR Buffer ( 1 bag diluted in 750 ml distilled water ) – Fat Red 7B ( 1 vial diluted in 100 ml of methanol ) – Lipo Spray – Lipotrol ( Control ) – Report Forms – Blotters – Glycerin – Sodium Hydroxyde 1N . ۳) METHOD – Wet the plates 30m in buffer – Sample volume : 5 µl of serum – Electrophoresis : 20 mn at 180 V . ۴) STAINING – Stock solution : dilute 1 vial of powder in one liter of methanol , mix for 3 – About 5 mn before the end of the migration , mix in a dish : 30 ml of stock – At the end of the staining time , gently rinse the plate under tap water . – Then put the plate in a plastic bag with some drops of water , close it ۵) SCANNING – Scan with your densitometer at 525 nm . – If you want to keep the plate : put it for 10 mn in a solution composed of 80
LIPOPROTEINS ELECTROPHORESIS ۱) ACCESSORIES – Power Supply – Chamber – Applicator Kit – Micro dispenser 0-10 μl – Staining Set and Rack – Oven I.O.D ۲) REAGENTS AND CONSUMABLES – Acetate plates – Ponceau S (1 bag diluted in 1000 ml distilled water) – Clear Aid – Serum controls (kemtols) – Report Forms – Blotters – Glacial acetic acid ۳) METHOD – Wet the plates 20 mn in buffer – Sample volume : 3 µl of serum – One center application ۴) STAINING – Ponceau S : 6 mn . – Destaining : 3 differents washes in 5 % acetic acid 3 mn each . – Deshydratation : 2 diffrents washes in pure methanol 2 mn each . – Clearing solution ………….. ۶۷ % pure methanol – At the end of the clearing time drain the plate vertically for about 1 mn . – Put the plate in an oven ( acetate side up ) for 10 mn at 50c 60c – Wipe the mylar side . ۵) SCANNING – Scan with your densitometer at 525 nm .
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